Proteomics in Practice

Proteomics in Practice

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Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.GE Healthcare Handbook: a€œAffinity Chromatography, Principles and Methodsa€ ( 2002) 18-1022-29. GE Healthcare Handbook: a€œFluorescence imaging: Principles and Methodsa€ (2002) 63-0035-28. GE Healthcare User Manual: a€œEttan Spot Pickeranbsp;...

Title:Proteomics in Practice
Author: Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker
Publisher:John Wiley & Sons - 2008-09-08

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